| 1.
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Explain microbial products and the uses of microbes in biotechnology.
(I)
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| 2.
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Explain the genetics of lambda phage by describing antitermination and
autogenous lysogeny. (II)
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| 3.
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Describe DNA-binding proteins and the significance of the
helix-turn-helix motif. (II)
|
| 4.
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Explain the processes of protein synthesis, localization, and
degradation. (III)
|
| 5.
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Describe the significance of signal peptides. (III)
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| 6.
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Describe gene expression, and apply this concept to the expression of
a hypothetical gene using the operon, regulon, sigma, and rho. (IV)
|
| 7.
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Explain basic enzymology through rate calculations and description of
different types of inhibitors. (V)
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| 8.
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Explain DNA replication. (V)
|
| 9.
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Explain PCR by applying the technique to generate DNA template for
sequencing. (VI)
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| 10.
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Conduct a homology search between student-prepared DNA and protein
sequences and sequences cataloged in the database. (VI)
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| 11.
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Describe the use of lacZ and phoA in biotechnology. (VII)
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| 12.
|
Perform enzyme assays. (VII)
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| 13.
|
Select a transformant that exhibits especially high expression of
?-galactosidase. (VII)
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| 14.
|
Describe several different purification protocols. (VIII)
|
| 15.
|
Explain chromatography. (VIII)
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| 16.
|
Purify ?-galactosidase using ammonia sulfate precipitation, gel
filtration chromatography and ion exchange chromatography. (VIII)
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| 17.
|
Follow purification steps with SDS-Polyacrylamide gel electrophoresis
and protein assays. (VIII)
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|