MCCCD Official Course Competencies:

BIO212AC  20016-99999

Biotechnology III

1.

Describe methodology and applications of gene therapy, including failures and successes. (I)

2.

Describe synthetic DNA and drugs. (I)

3.

Describe the function of an antigen, comparing antibody and cell-mediated responses, and describing the genetic mechanism responsible for the immune response. (II)

4.

Conduct immunoassays using purified antibody and run SDS-PAGE to provide evidence of purity. (II)

5.

Complete dot blots of crude antisera produced against ?- galactosidase. (II)

6.

Develop a competition ELISA, establishing a standard curve to test the validity of the assay by exchange with other students. (II)

7.

Describe methodology and applications of plant biotechnology. (III)

8.

Describe public fears and concerns about plant biotechnology by presenting informed opinions in a debate format. (III)

9.

Describe methodology and applications of animal biotechnology. (IV)

10.

Describe regulations governing control of transgenic animals. (IV)

11.

Describe methodology and applications of biotechnology in marine biology. (V)

12.

Purify a gene from a representative aquatic organism by preparing a genomic library from Vibrio fischeri, selecting clones that glow most intensely, conducting a southern blot to confirm clone identity, and purifying plasmid DNA. (V)

13.

Describe the function of ATCC, growth media and supplementation, and growth conditions. (VI)

14.

Transform a eukaryotic cell by electroporation. (VI)

15.

Monitor the expression of a gene in a transformed, cultured cell line. (VI)

MCCCD Official Course Outline:

BIO212AC  20016-99999

Biotechnology III

I. Medical Biotechnology

A. Genes that cause disease

B. Gene therapy

C. Gene delivery methods

D. Regulation of gene therapy trials

E. Viral delivery

F. Gene therapy models

G. Commercialization of gene therapy

H. Genetic screening, counseling, and social implications of treating genetic disease

I. Synthetic DNA

J. Tissue engineering

K. Synthetic drugs

L. Xenotransplantation

II. The Immune Response and the Biotechnologist

A. Major histocompatibility complex (MHC) genes

B. Immunoglobulin genes

C. Diversity of germline information

D. Recombination generated deletions and rearrangements

E. Allelic exclusion

F. DNA recombination and class switching

G. B cell development and memory

H. T-cell receptors and immunoglobulins

I. Immunoassays and antibody titer determinations

J. Dot blots, western blot hybridization, and ELISA

K. Techniques for handling and purifying antibodies

III. Plant Biotechnology

A. Plant tissue culture and applications

B. Applications of plant genetic engineering

C. Agricultural genetic engineering and public concerns

D. Plant genetic engineering and the future

IV. Animal Biotechnology

A. Gene transfer methods in animals

B. Transgenic animals

C. Animal diseases

D. Biotechnology and conservation biology

E. Regulation of transgenic animals

F. Patenting genetically engineered animals

V. Marine Biotechnology

A. Aquaculture

B. Marine animal health

C. Fuels from algae

D. Anticancer and antiviral compounds

E. Marine toxins

F. Transgenic fish

G. A genomic library from Vibrio fischeri

H. The luxA gene

VI. Cells in Culture

A. What is cell culture?

B. Growth requirements of eukaryotic cells in culture

C. The American Tissue Culture Collection

D. Electroporation and transformation of a eukaryotic cell

E. Gene expression in a transformed, cultured cell line