Maricopa Community Colleges  AGS255   20054-99999 
Official Course Description: MCCCD Approval: 03/22/05
AGS255 20054-99999 L+L 3 Credit(s) 5 Period(s)
Plant Tissue Culture
Plant tissue culture techniques for cloning, reproduction, and manufacturing including media mixing, cell growth and development, aseptic transfer, manipulation, observation, and documentation. Prerequisites: AGS164.
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MCCCD Official Course Competencies:
 
AGS255   20054-99999 Plant Tissue Culture
1. Describe the history of the biotechnology industry. (I)
2. Compare and contrast the two major areas of biotechnology. (I)
3. Design and apply a laboratory safety plan utilizing procedures associated with biotechnology. (II)
4. Apply knowledge of plant growth and development to reproduce plants. (III)
5. Analyze the effects of plant growth hormones. (III)
6. Create viable plant tissue culture media. (IV)
7. Operate tissue culture lab equipment. (IV)
8. Utilize aseptic technique to produce plant tissue cultures. (V)
9. Research and design plant tissue culture media. (VI)
10. Operate microscopic lab equipment. (VII)
11. Complete micro-propagation experiments. (VIII)
12. Analyze and project cell cultures. (IX)
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MCCCD Official Course Outline:
 
AGS255   20054-99999 Plant Tissue Culture
    I. Biotechnology Introduction
        A. Brief history
        B. Plant propagation
        C. Tissue culture
        D. Pros/cons; advantages/disadvantages of Plant Tissue Culture (PTC)
      II. Biosafety
          A. Chemical "right to know" – Material Safety Data Sheets (MSDS)
          B. Chemical labeling
          C. Introduction to occupational exposure limits/lethal concentration (LC)/lethal dose (LD)/etc.
          D. Regulations on transport, storage, disposal of chemical/biohazardous materials/waste
          E. Chemical hazards – acids, bases, compressed gas
          F. Biosafety levels – 1, 2, 3, 4
          G. Personal protective clothing and equipment
        III. Plant growth and development review
            A. Parts of the plant
            B. Cell types
            C. Asexual vs. sexual reproduction
            D. Effects of hormones
            E. Common plant cell types – Arabidopsis, Venus Flytrap, African Violet, etc.
          IV. Media and solution preparation
              A. Units of concentration and volume
              B. Basic mathematical calculations for lab sciences
              C. Buffers and pH
              D. Autoclaving and Filter sterilization
              E. Lab equipment operation
            V. Aseptic Technique
                A. Theory
                B. Use of laminar flow/biosafety hoods
                C. Hood setup
                D. Plant sterilization
                E. Evaluating contamination
              VI. Plant Tissue Culture Media
                  A. Media types
                  B. Plant hormones
                  C. Making media
                VII. Microscopy
                    A. Basic microscope usage and cell staining
                    B. Hemocytometer use to count viable cells
                  VIII. Micro propagation
                      A. From Leaf/stem/root cuttings
                      B. From callus
                      C. Micro propagate shooting plants
                      D. Micro propagate rooting plants
                    IX. Cell culture maintenance
                        A. Monitoring clones/explants
                        B. Changing media types
                        C. Hardening off explants (prep for greenhouse/propagation)
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